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NXF1 Monoclonal antibody

NXF1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C2H1

Cat no : 67914-1-Ig

Print datasheet

Synonyms

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

验证数据展示

  • WB analysis using 67914-1-Ig

    WB analysis using 67914-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67914-1-Ig (NXF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67914-1-Ig

    IF Staining of HEK-293 using 67914-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NXF1 antibody (67914-1-Ig, Clone: 2C2H1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, LNcaP cells, Hela cells, HepG2 cells, K-562 cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy NXF1 Ready-To-Use IHC Kit. NXF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67914-1-Ig targets NXF1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen NXF1 fusion protein Ag27329 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear RNA export factor 1
Synonyms DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein
Calculated Molecular Weight 70 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC004904
Gene Symbol NXF1
Gene ID (NCBI) 10482
RRIDAB_2918668
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UBU9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.

实验方案

Product Specific Protocols
WB protocol for NXF1 antibody 67914-1-IgDownload protocol
IF protocol for NXF1 antibody 67914-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols