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验证数据展示

  • WB analysis using 67914-1-Ig

    WB analysis using 67914-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67914-1-Ig (NXF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67914-1-Ig

    IF Staining of HEK-293 using 67914-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NXF1 antibody (67914-1-Ig, Clone: 2C2H1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

NXF1 Monoclonal antibody

NXF1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67914-1-Ig

产品说明书

CloneNo. 2C2H1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, LNcaP cells, Hela cells, HepG2 cells, K-562 cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy NXF1 Ready-To-Use IHC Kit. NXF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67914-1-Ig targets NXF1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 NXF1 fusion protein Ag27329 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear RNA export factor 1
别名 DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein
计算分子量 70 kDa
观测分子量67 kDa
GenBank蛋白编号BC004904
基因名称 NXF1
Gene ID (NCBI) 10482
RRIDAB_2918668
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UBU9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.

实验方案

Product Specific Protocols
WB protocol for NXF1 antibody 67914-1-IgDownload protocol
IF protocol for NXF1 antibody 67914-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols