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NXF1 Monoclonal antibody, PBS Only

NXF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C2H1

Cat no : 67914-1-PBS

Print datasheet

Synonyms

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

验证数据展示

  • WB analysis using 67914-1-Ig (same clone as 67914-1-PBS)

    WB analysis using 67914-1-Ig (same clone as 67914-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67914-1-Ig (NXF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67914-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67914-1-Ig (same clone as 67914-1-PBS)

    IF Staining of HEK-293 using 67914-1-Ig (same clone as 67914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NXF1 antibody (67914-1-Ig, Clone: 2C2H1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67914-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NXF1 Ready-To-Use IHC Kit. NXF1 primary antibody included.

产品信息

67914-1-PBS targets NXF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen NXF1 fusion protein Ag27329 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear RNA export factor 1
Synonyms DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein
Calculated Molecular Weight 70 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC004904
Gene Symbol NXF1
Gene ID (NCBI) 10482
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UBU9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.