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验证数据展示

  • IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

    IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    IF Staining of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    IP experiment of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

    IP result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • WB analysis using 16128-1-AP (same clone as 16128-1-PBS)

    WB analysis using 16128-1-AP (same clone as 16128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NXN Polyclonal antibody, PBS Only

NXN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16128-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

16128-1-PBS targets NXN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NXN fusion protein Ag9113 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleoredoxin
别名
计算分子量 435 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC009327
基因名称 NXN
Gene ID (NCBI) 64359
RRIDAB_2158114
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6DKJ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.