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Nectin-2/CD112 Recombinant antibody

Nectin-2/CD112 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84460-5-RR

产品说明书

CloneNo. 241725D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

Nectin-2, Nectin 2, HVEB, Herpesvirus entry mediator B, Herpes virus entry mediator B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-29 cells, HAP1 cells, A549 cells, MDA-MB-231 cells
Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy Nectin-2/CD112 Ready-To-Use IHC Kit. Nectin-2/CD112 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84460-5-RR targets Nectin-2/CD112 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poliovirus receptor-related 2 (herpesvirus entry mediator B)
别名 Nectin-2, Nectin 2, HVEB, Herpesvirus entry mediator B, Herpes virus entry mediator B
计算分子量58 kDa
观测分子量65-75 kDa
GenBank蛋白编号NM_001042724.2
基因名称 Nectin-2/CD112
Gene ID (NCBI) 5819
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92692-2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

实验方案

Product Specific Protocols
WB protocol for Nectin-2/CD112 antibody 84460-5-RRDownload protocol
IF protocol for Nectin-2/CD112 antibody 84460-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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