验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, HAP1 cells, A549 cells, MDA-MB-231 cells |
Positive IF/ICC detected in | K-562 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84460-5-RR targets Nectin-2/CD112 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | poliovirus receptor-related 2 (herpesvirus entry mediator B) |
Synonyms | Nectin-2, Nectin 2, HVEB, Herpesvirus entry mediator B, Herpes virus entry mediator B |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 65-75 kDa |
GenBank Accession Number | NM_001042724.2 |
Gene Symbol | Nectin-2/CD112 |
Gene ID (NCBI) | 5819 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q92692-2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.
实验方案
Product Specific Protocols | |
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WB protocol for Nectin-2/CD112 antibody 84460-5-RR | Download protocol |
IF protocol for Nectin-2/CD112 antibody 84460-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |