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Nectin-4/PVRL4 Recombinant antibody

Nectin-4/PVRL4 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

240009E2

Cat No : 83223-6-RR

Print datasheet

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4

验证数据展示

  • WB analysis using 83223-6-RR

    WB analysis using 83223-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83223-6-RR (Nectin-4/PVRL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83223-6-RR

    FC experiment of MCF-7 using 83223-6-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Nectin-4/PVRL4 Recombinant antibody (83223-6-RR, Clone: 240009E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman placenta tissue, A2780 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83223-6-RR is Nectin-4/PVRL4 Fusion Protein expressed in E. coli.

Tested Applications WB, FC, ELISA Application Description
Tested Reactivity Human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name poliovirus receptor-related 4
Synonyms Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC010423
Gene Symbol PVRL4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96NY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

实验方案

Product Specific Protocols
WB protocol for Nectin-4/PVRL4 antibody 83223-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols