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Nectin-4/PVRL4 Recombinant antibody, PBS Only

Nectin-4/PVRL4 Recombinant Antibody for WB, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240009E2

Cat No : 83223-6-PBS

Print datasheet

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4

验证数据展示

  • WB analysis using 83223-6-RR (same clone as 83223-6-PBS)

    WB analysis using 83223-6-RR (same clone as 83223-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83223-6-RR (Nectin-4/PVRL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83223-6-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83223-6-RR (same clone as 83223-6-PBS)

    FC experiment of MCF-7 using 83223-6-RR (same clone as 83223-6-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Nectin-4/PVRL4 Recombinant antibody (83223-6-RR, Clone: 240009E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83223-6-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.

产品信息

The immunogen of 83223-6-PBS is Nectin-4/PVRL4 Fusion Protein expressed in E. coli.

Tested Applications WB, FC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name poliovirus receptor-related 4
Synonyms Ig superfamily receptor LNIR, LNIR, nectin 4, poliovirus receptor related 4, PRR4, PVRL4
Calculated Molecular Weight 510 aa, 55 kDa
GenBank Accession NumberBC010423
Gene Symbol PVRL4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96NY8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).