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Nesprin 3 Monoclonal antibody, PBS Only

Nesprin 3 Monoclonal Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D1G12

Cat no : 68420-1-PBS

Print datasheet

Synonyms

C14orf49, FLJ25605, Nesprin 3

验证数据展示

  • WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

    WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

    IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

产品信息

68420-1-PBS targets Nesprin 3 in WB, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Nesprin 3 fusion protein Ag26012 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromosome 14 open reading frame 49
Synonyms C14orf49, FLJ25605, Nesprin 3
Observed Molecular Weight110 kDa
GenBank Accession NumberBC146604
Gene Symbol Nesprin 3
Gene ID (NCBI) 161176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6ZMZ3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).