• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68420-1-Ig

    WB analysis using 68420-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 68420-1-Ig

    IF Staining of mouse testis using 68420-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68420-1-Ig

产品说明书

CloneNo. 1D1G12

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF-P, ELISA

别名

C14orf49, FLJ25605, Nesprin 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, C2C12 cells, PC-12 cells, rat testis tissue
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68420-1-Ig targets Nesprin 3 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Nesprin 3 fusion protein Ag26012 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 49
别名 C14orf49, FLJ25605, Nesprin 3
观测分子量110 kDa
GenBank蛋白编号BC146604
基因名称 Nesprin 3
Gene ID (NCBI) 161176
RRIDAB_3085135
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6ZMZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

实验方案

Product Specific Protocols
WB protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
IF protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols