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CoraLite® Plus 488-conjugated Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D1G12

Cat No : CL488-68420

Print datasheet

Synonyms

C14orf49, FLJ25605, Nesprin 3

验证数据展示

  • IF Staining of mouse testis using CL488-68420

    IF Staining of mouse testis using CL488-68420

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 488 Nesprin 3 antibody (CL488-68420, Clone: 1D1G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 68420-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68420 targets Nesprin 3 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, mouse, rat
Immunogen Nesprin 3 fusion protein Ag26012 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromosome 14 open reading frame 49
Synonyms C14orf49, FLJ25605, Nesprin 3
Observed Molecular Weight110 kDa
GenBank Accession NumberBC146604
Gene Symbol C14orf49
Gene ID (NCBI) 161176
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6ZMZ3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Nesprin 3 antibody CL488-68420Download protocol
Standard Protocols
Click here to view our Standard Protocols