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验证数据展示

  • FC experiment of NIH/3T3 using CL488-68420

    FC experiment of NIH/3T3 using CL488-68420

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Nesprin 3 Monoclonal antibody (CL488-68420, Clone:1D1G12)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of mouse testis using CL488-68420

    IF Staining of mouse testis using CL488-68420

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 488 Nesprin 3 antibody (CL488-68420, Clone: 1D1G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-68420

产品说明书

CloneNo. 1D1G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

LINC00341, KASH3, KASH domain-containing protein 3, C14orf49, C14orf139

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 68420-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68420 targets Nesprin 3 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Nesprin 3 fusion protein Ag26012 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 49
别名 LINC00341, KASH3, KASH domain-containing protein 3, C14orf49, C14orf139
观测分子量110 kDa
GenBank蛋白编号BC146604
基因名称 Nesprin 3
Gene ID (NCBI) 161176
RRIDAB_3673011
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6ZMZ3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Nesprin 3 antibody CL488-68420Download protocol
Standard Protocols
Click here to view our Standard Protocols