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Nestin Polyclonal antibody, PBS Only

Nestin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 19483-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IF Staining of U-251 using 19483-1-AP (same clone as 19483-1-PBS)

    IF Staining of U-251 using 19483-1-AP (same clone as 19483-1-PBS)

    Immunofluorescent analysis of U-251 cells using 19483-1-AP (Nestin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • WB analysis using 19483-1-AP (same clone as 19483-1-PBS)

    WB analysis using 19483-1-AP (same clone as 19483-1-PBS)

    Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 19483-1-AP (same clone as 19483-1-PBS)

    IHC staining of human gliomas using 19483-1-AP (same clone as 19483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Nestin antibody included
  • IHC staining of human kidney using 19483-1-AP (same clone as 19483-1-PBS)

    IHC staining of human kidney using 19483-1-AP (same clone as 19483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Nestin antibody included
  • IF Staining of SH-SY5Y using 19483-1-AP (same clone as 19483-1-PBS)

    IF Staining of SH-SY5Y using 19483-1-AP (same clone as 19483-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 19483-1-AP (same clone as 19483-1-PBS)

    FC experiment of SH-SY5Y using 19483-1-AP (same clone as 19483-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Nestin Polyclonal antibody (19483-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19483-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Nestin Ready-To-Use IHC Kit. Nestin primary antibody included.

产品信息

19483-1-PBS targets Nestin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nestin
Synonyms
Calculated Molecular Weight 177 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_10644440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48681
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).