验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human kidney tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | SH-SY5Y cells, U-251 cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19483-1-AP targets Nestin in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey, zebrafish |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nestin |
| 别名 | NES |
| 计算分子量 | 177 kDa |
| 观测分子量 | 170-180 kDa |
| GenBank蛋白编号 | NM_006617 |
| 基因名称 | Nestin |
| Gene ID (NCBI) | 10763 |
| RRID | AB_10644440 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48681 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Nestin antibody 19483-1-AP | Download protocol |
| IHC protocol for Nestin antibody 19483-1-AP | Download protocol |
| IF protocol for Nestin antibody 19483-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
Int J Surg Stereotactically intracerebral transplantation of neural stem cells for ischemic stroke attenuated inflammatory responses and promoted neurogenesis: an experimental study with monkeys | ||
J Biomed Sci Genetic and pharmacologic p32-inhibition rescue CHCHD2-linked Parkinson's disease phenotypes in vivo and in cell models | ||
Neuro Oncol TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression. | ||
Redox Biol MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway. | ||
Acta Biomater Induced pluripotent stem cell-derived neural stem cells promote bone formation in mice with calvarial defects |
