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验证数据展示

  • IF Staining of rat brain using CL594-19483

    IF Staining of rat brain using CL594-19483

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 Nestin antibody (CL594-19483) at dilution of 1:200. DAPI (blue).

  • IF Staining of human kidney using CL594-19483

    IF Staining of human kidney using CL594-19483

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 Nestin antibody (CL594-19483) at dilution of 1:200.

CoraLite®594-conjugated Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF-P
Cat No. CL594-19483

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

FLJ21841, Nbla00170, NES, nestin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inrat brain tissue, human kidney tissue
Planning an IHC experiment? We recommend our IHCeasy Nestin Ready-To-Use IHC Kit. Nestin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19483-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-19483 targets Nestin in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nestin
别名 FLJ21841, Nbla00170, NES, nestin
计算分子量 177 kDa
观测分子量 170-180 kDa
GenBank蛋白编号NM_006617
基因名称 Nestin
Gene ID (NCBI) 10763
RRIDAB_2919852
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48681
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

实验方案

Product Specific Protocols
IF protocol for CL594 Nestin antibody CL594-19483Download protocol
Standard Protocols
Click here to view our Standard Protocols