验证数据展示
产品信息
29285-1-PBS targets Nestin in WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Nestin fusion protein Ag29865 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | nestin |
别名 | NES |
计算分子量 | 177 kDa |
观测分子量 | 240-280 kDa |
GenBank蛋白编号 | NM_006617 |
基因名称 | Nestin |
Gene ID (NCBI) | 10763 |
RRID | AB_2918268 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P48681 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).