验证数据展示
产品信息
29285-1-PBS targets Nestin in WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Nestin fusion protein Ag29865 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nestin |
Synonyms | NES |
Calculated Molecular Weight | 177 kDa |
Observed Molecular Weight | 240-280 kDa |
GenBank Accession Number | NM_006617 |
Gene Symbol | Nestin |
Gene ID (NCBI) | 10763 |
RRID | AB_2918268 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P48681 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).