Neurofibromin 1 Recombinant antibody
Neurofibromin 1 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240540F2
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, PC-12 cell, NIH/3T3 cells, HEK-293T cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy NF1 Ready-To-Use IHC Kit. NF1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 83620-5-RR is Neurofibromin 1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | Neurofibromin 1 fusion protein Ag25799 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | neurofibromin 1 |
| Synonyms | Neurofibromin, NF1, NF-1, neurofibromin 1, NF 1, 240540F2 |
| Calculated Molecular Weight | 319 kDa |
| Observed Molecular Weight | 250-280 kDa |
| GenBank Accession Number | M60915 |
| Gene Symbol | Neurofibromin 1 |
| Gene ID (NCBI) | 4763 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21359 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neurofibromin 1 antibody 83620-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |