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Neurofibromin 1 Recombinant antibody

Neurofibromin 1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240540F2

Cat no : 83620-5-RR

Print datasheet

Synonyms

Neurofibromin, NF1, NF-1, NF 1, 240540F2

验证数据展示

  • WB analysis using 83620-5-RR

    WB analysis using 83620-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83620-5-RR

    FC experiment of HeLa using 83620-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83620-5-RR

    Affinity and Kinetic Characterization of 83620-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA431 cells, PC-12 cell, NIH/3T3 cells, HEK-293T cells, HeLa cells
Positive FC (Intra) detected inHeLa cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy NF1 Ready-To-Use IHC Kit. NF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83620-5-RR is Neurofibromin 1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Neurofibromin 1 fusion protein Ag25799 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neurofibromin 1
Synonyms Neurofibromin, NF1, NF-1, NF 1, 240540F2
Calculated Molecular Weight 319 kDa
Observed Molecular Weight250-280 kDa
GenBank Accession NumberM60915
Gene Symbol Neurofibromin 1
Gene ID (NCBI) 4763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21359
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

实验方案

Product Specific Protocols
WB protocol for Neurofibromin 1 antibody 83620-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols