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Neurofibromin Recombinant antibody, PBS Only (Capture)

Neurofibromin Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240540F2

Cat no : 83620-5-PBS

Print datasheet

Synonyms

NF1, NF-1, NF 1, neurofibromin 1, 240540F2

验证数据展示

  • WB analysis using 83620-5-RR (same clone as 83620-5-PBS)

    WB analysis using 83620-5-RR (same clone as 83620-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83620-5-RR (same clone as 83620-5-PBS)

    FC experiment of HeLa using 83620-5-RR (same clone as 83620-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00570-2

    Cytometric bead array standard curve of MP00570-2

    Cytometric bead array standard curve of MP00570-2, Neurofibromin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83620-5-PBS. Detection antibody: 83620-4-PBS. Standard: Ag25799. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83620-5-RR

    Affinity and Kinetic Characterization of 83620-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy NF1 Ready-To-Use IHC Kit. NF1 primary antibody included.

产品信息

83620-5-PBS targets Neurofibromin as part of a matched antibody pair:

MP00570-2: 83620-5-PBS capture and 83620-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Neurofibromin fusion protein Ag25799 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neurofibromin 1
Synonyms NF1, NF-1, NF 1, neurofibromin 1, 240540F2
Calculated Molecular Weight 319 kDa
Observed Molecular Weight250-280 kDa
GenBank Accession NumberM60915
Gene Symbol Neurofibromin 1
Gene ID (NCBI) 4763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21359
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C