验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, rat heart tissue, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84429-5-RR targets Neuropilin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | neuropilin 1 |
别名 | NRP1, Neuropilin-1, CD304, BDCA4, 241812G11 |
观测分子量 | 130-140 kDa |
GenBank蛋白编号 | BC007533 |
基因名称 | Neuropilin 1 |
Gene ID (NCBI) | 8829 |
RRID | AB_3671956 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | O14786 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Neuropilin 1 antibody 84429-5-RR | Download protocol |
IHC protocol for Neuropilin 1 antibody 84429-5-RR | Download protocol |
IF protocol for Neuropilin 1 antibody 84429-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |