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Neuroserpin Monoclonal antibody, PBS Only

Neuroserpin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4C6

Cat no : 66997-1-PBS

Print datasheet

Synonyms

DKFZp781N13156, neuroserpin, Peptidase inhibitor 12, PI 12, PI12, Serpin I1, SERPINI1

验证数据展示

  • WB analysis of pig brain using 66997-1-Ig (same clone as 66997-1-PBS)

    WB analysis of pig brain using 66997-1-Ig (same clone as 66997-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66997-1-Ig (SERPINI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

    IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

    IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 66997-1-Ig (same clone as 66997-1-PBS)

    IF Staining of U-251 using 66997-1-Ig (same clone as 66997-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Neuroserpin antibody (66997-1-Ig, Clone: 1G4C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

产品信息

66997-1-PBS targets Neuroserpin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, pig, rat
Immunogen Neuroserpin fusion protein Ag28636 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade I (neuroserpin), member 1
Synonyms DKFZp781N13156, neuroserpin, Peptidase inhibitor 12, PI 12, PI12, Serpin I1, SERPINI1
Calculated Molecular Weight 410 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC018043
Gene Symbol Neuroserpin
Gene ID (NCBI) 5274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99574
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.