Neuroserpin Monoclonal antibody
Neuroserpin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, pig, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1G4C6
验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue |
| Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
66997-1-Ig targets Neuroserpin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, pig, rat |
| Cited Reactivity | mouse |
| Immunogen | Neuroserpin fusion protein Ag28636 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | serpin peptidase inhibitor, clade I (neuroserpin), member 1 |
| Synonyms | DKFZp781N13156, neuroserpin, Peptidase inhibitor 12, PI 12, PI12, Serpin I1, SERPINI1 |
| Calculated Molecular Weight | 410 aa, 47 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC018043 |
| Gene Symbol | Neuroserpin |
| Gene ID (NCBI) | 5274 |
| RRID | AB_2882314 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q99574 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| IHC protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| IF protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|