验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue |
| Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
66997-1-Ig targets Neuroserpin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, pig, rat |
| 文献引用反应性 | mouse |
| 免疫原 | Neuroserpin fusion protein Ag28636 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade I (neuroserpin), member 1 |
| 别名 | DKFZp781N13156, neuroserpin, Peptidase inhibitor 12, PI 12, PI12, Serpin I1, SERPINI1 |
| 计算分子量 | 410 aa, 47 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC018043 |
| 基因名称 | Neuroserpin |
| Gene ID (NCBI) | 5274 |
| RRID | AB_2882314 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99574 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| IHC protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| IF protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |