验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66997 targets Neuroserpin in IF/ICC applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat, pig |
| 免疫原 | Neuroserpin fusion protein Ag28636 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade I (neuroserpin), member 1 |
| 别名 | Serpin I1, PI-12, PI12, PI 12, Peptidase inhibitor 12 |
| 计算分子量 | 410 aa, 47 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC018043 |
| 基因名称 | Neuroserpin |
| Gene ID (NCBI) | 5274 |
| RRID | AB_3672932 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99574 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Neuroserpin antibody CL488-66997 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |