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验证数据展示

  • WB analysis using 16634-1-AP (same clone as 16634-1-PBS)

    WB analysis using 16634-1-AP (same clone as 16634-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16634-1-AP (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 16634-1-AP (same clone as 16634-1-PBS)

    IHC staining of human liver cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 16634-1-AP (same clone as 16634-1-PBS)

    IHC staining of human liver cancer using 16634-1-AP (same clone as 16634-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16634-1-AP (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16634-1-AP (same clone as 16634-1-PBS)

    IF Staining of HepG2 using 16634-1-AP (same clone as 16634-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OCIAD1 antibody (16634-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 16634-1-AP (same clone as 16634-1-PBS)

    IP experiment of HeLa using 16634-1-AP (same clone as 16634-1-PBS)

    IP result of anti-OCIAD1 (IP:16634-1-AP, 4ug; Detection:16634-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16634-1-PBS in a different storage buffer formulation.

OCIAD1 Polyclonal antibody, PBS Only

OCIAD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16634-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

TPA018, Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

16634-1-PBS targets OCIAD1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 OCIAD1 fusion protein Ag9977 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OCIA domain containing 1
别名 TPA018, Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA
计算分子量 28 kDa
观测分子量 29-35 kDa
GenBank蛋白编号BC003409
基因名称 OCIAD1
Gene ID (NCBI) 54940
RRIDAB_2156297
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NX40
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).