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CoraLite® Plus 488-conjugated OCIAD1 Monoclonal antibody
OCIAD1 Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC, IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C10C3
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 66698-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66698 targets OCIAD1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | OCIAD1 fusion protein Ag9977 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | OCIA domain containing 1 |
| Synonyms | Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC003409 |
| Gene Symbol | OCIAD1 |
| Gene ID (NCBI) | 54940 |
| RRID | AB_2934487 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NX40 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 OCIAD1 antibody CL488-66698 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |