OCT4 Monoclonal antibody

OCT4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60242-1-Ig

产品说明书

CloneNo. 1C4B6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

POU5F1, Octamer-binding protein 4, Octamer-binding protein 3, Oct-4, Oct-3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
HRP
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, Caki-2 cells, human brain tissue, NIH/3T3 cells, HT-1376 cells, NCCIT cells, L02 cells, Neuro-2a cells, mMSCs cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60242-1-Ig targets OCT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 OCT4 fusion protein Ag1794 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 POU class 5 homeobox 1
别名 POU5F1, Octamer-binding protein 4, Octamer-binding protein 3, Oct-4, Oct-3
计算分子量 39 kDa
观测分子量 50-52 kDa
GenBank蛋白编号BC020712
基因名称 POU5F1
Gene ID (NCBI) 5460
RRIDAB_2881364
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01860
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).



实验方案

Product Specific Protocols
WB protocol for OCT4 antibody 60242-1-IgDownload protocol
IHC protocol for OCT4 antibody 60242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
humanWB

J Hematol Oncol

A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.

Authors - Shuiqing Liu
humanWB

Theranostics

Sulforaphane Inhibits the Acquisition of Tobacco Smoke-Induced Lung Cancer Stem Cell-Like Properties via the IL-6/ΔNp63α/Notch Axis.

Authors - Chunfeng Xie
humanWB

Cancer Commun (Lond)

KDELR2 promotes breast cancer proliferation via HDAC3-mediated cell cycle progression.

Authors - Haoran Wei
humanWB

Front Oncol

PIWIL1 Drives Chemoresistance in Multiple Myeloma by Modulating Mitophagy and the Myeloma Stem Cell Population.

Authors - Yajun Wang
humanIF

Stem Cell Res

Generation of a human induced pluripotent stem cell line (INNDSUi003-A) derived from patient with Becker muscular dystrophy (BMD).

Authors - Xinbo Ji
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