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验证数据展示

  • WB analysis of HEK-293 using HRP-60242

    WB analysis of HEK-293 using HRP-60242

    HEK-293 cells were subjected to SDS PAGE followed by western blot with HRP-60242 (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using HRP-60242

    WB analysis using HRP-60242

    Various lysates were subjected to SDS PAGE followed by western blot with HRP-60242 (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HRP-conjugated OCT4 Monoclonal antibody

OCT4 Monoclonal Antibody for WB
Cat No. HRP-60242

产品说明书

CloneNo. 1C4B6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB

别名

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  HRP
规格: 

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经过测试的应用

Positive WB detected inHEK-293 cells, HT-29 cells, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-60242 targets OCT4 in WB applications and shows reactivity with human, mouse samples.

经测试应用 WB Application Description
经测试反应性 human, mouse
免疫原 OCT4 fusion protein Ag1794 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 POU class 5 homeobox 1
别名 Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1
计算分子量 39 kDa
观测分子量 50-52 kDa
GenBank蛋白编号BC020712
基因名称 POU5F1
Gene ID (NCBI) 5460
RRIDAB_2883831
偶联类型 HRP
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ01860
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).



实验方案

Product Specific Protocols
WB protocol for HRP OCT4 antibody HRP-60242Download protocol
Standard Protocols
Click here to view our Standard Protocols