验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HUVEC cells, human placenta tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67336-1-Ig targets ODC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | ODC1 fusion protein Ag10927 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ornithine decarboxylase 1 |
| 别名 | ODC, EC:4.1.1.17, 2A1E3 |
| 计算分子量 | 461 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC025296 |
| 基因名称 | ODC1 |
| Gene ID (NCBI) | 4953 |
| RRID | AB_2882594 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11926 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ODC1 antibody 67336-1-Ig | Download protocol |
| IF protocol for ODC1 antibody 67336-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
