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CoraLite® Plus 488-conjugated ODF2 Polyclonal antibody
ODF2 Polyclonal Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
For other applications, we recommend the unconjugated version of this antibody, 12058-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12058 targets ODF2 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | ODF2 fusion protein Ag2693 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | outer dense fiber of sperm tails 2 |
| Synonyms | Cenexin, FLJ44866, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2 |
| Calculated Molecular Weight | 763 aa, 89 kDa |
| Observed Molecular Weight | 67-100 kDa |
| GenBank Accession Number | BC010629 |
| Gene Symbol | ODF2 |
| Gene ID (NCBI) | 4957 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5BJF6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 ODF2 antibody CL488-12058 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |