验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12058 targets ODF2 in WB applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ODF2 fusion protein Ag2693 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | outer dense fiber of sperm tails 2 |
| 别名 | Cenexin, FLJ44866, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2 |
| 计算分子量 | 763 aa, 89 kDa |
| 观测分子量 | 67-100 kDa |
| GenBank蛋白编号 | BC010629 |
| 基因名称 | ODF2 |
| Gene ID (NCBI) | 4957 |
| RRID | AB_3083914 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5BJF6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 ODF2 antibody CL488-12058 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |