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验证数据展示

  • IF Staining of HepG2 using CL488-66285

    IF Staining of HepG2 using CL488-66285

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:200, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated OGDH Monoclonal antibody

OGDH Monoclonal Antibody for IF/ICC
Cat No. CL488-66285

产品说明书

CloneNo. 1H7A2

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

IF/ICC

别名

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, L02 cells
Planning an IHC experiment? We recommend our IHCeasy OGDH Ready-To-Use IHC Kit. OGDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66285-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66285 targets OGDH in IF/ICC applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, rat
免疫原 OGDH fusion protein Ag7484 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
别名 E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1
计算分子量 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC004964
基因名称 OGDH
Gene ID (NCBI) 4967
RRIDAB_2883303
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ02218
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 OGDH antibody CL488-66285Download protocol
Standard Protocols
Click here to view our Standard Protocols