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CoraLite® Plus 488-conjugated OGDH Monoclonal antibody

OGDH Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7A2

Cat no : CL488-66285

Print datasheet

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

验证数据展示

  • IF Staining of HepG2 using CL488-66285

    IF Staining of HepG2 using CL488-66285

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 OGDH antibody (CL488-66285, Clone: 1H7A2 ) at dilution of 1:200, CL594-phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells, L02 cells
Planning an IHC experiment? We recommend our IHCeasy OGDH Ready-To-Use IHC Kit. OGDH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66285-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66285 targets OGDH in IF/ICC applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat
Immunogen OGDH fusion protein Ag7484 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Synonyms E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
RRIDAB_2883303
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02218
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 OGDH antibody CL488-66285Download protocol
Standard Protocols
Click here to view our Standard Protocols