验证数据展示
经过测试的应用
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | Caco-2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82981-1-RR targets OGDHL in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IF/ICC, FC (Intra), ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | OGDHL fusion protein Ag10794 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | oxoglutarate dehydrogenase-like |
| Synonyms | KIAA1290, EC:1.2.4.2, Alpha-ketoglutarate dehydrogenase-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1-like |
| Calculated Molecular Weight | 1010 aa, 115 kDa |
| GenBank Accession Number | BC026320 |
| Gene Symbol | OGDHL |
| Gene ID (NCBI) | 55753 |
| RRID | AB_3670730 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9ULD0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for OGDHL antibody 82981-1-RR | Download protocol |
| IF protocol for OGDHL antibody 82981-1-RR | Download protocol |
| FC protocol for OGDHL antibody 82981-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |