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OGDHL Recombinant antibody

OGDHL Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230228B2

Cat no : 82981-1-RR

Print datasheet

Synonyms

KIAA1290, EC:1.2.4.2, Alpha-ketoglutarate dehydrogenase-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1-like

验证数据展示

  • IF Staining of U2OS using 82981-1-RR

    IF Staining of U2OS using 82981-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using OGDHL antibody (82981-1-RR, Clone: 230228B2 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IHC staining of human liver using 82981-1-RR

    IHC staining of human liver using 82981-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 82981-1-RR

    IHC staining of human liver using 82981-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82981-1-RR (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Caco-2 using 82981-1-RR

    FC experiment of Caco-2 using 82981-1-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug OGDHL Recombinant antibody (82981-1-RR, Clone:230228B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82981-1-RR

    Affinity and Kinetic Characterization of 82981-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82981-1-RR against Human OGDHL were performed. The affinity constant is 4.98 nM.

经过测试的应用

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82981-1-RR targets OGDHL in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen OGDHL fusion protein Ag10794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name oxoglutarate dehydrogenase-like
Synonyms KIAA1290, EC:1.2.4.2, Alpha-ketoglutarate dehydrogenase-like, 2-oxoglutarate dehydrogenase-like, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1-like
Calculated Molecular Weight 1010 aa, 115 kDa
GenBank Accession NumberBC026320
Gene Symbol OGDHL
Gene ID (NCBI) 55753
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9ULD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for OGDHL antibody 82981-1-RRDownload protocol
IF protocol for OGDHL antibody 82981-1-RRDownload protocol
FC protocol for OGDHL antibody 82981-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols