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验证数据展示

  • WB analysis using CL488-66823

    WB analysis using CL488-66823

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66823 (OGT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated OGT Monoclonal antibody

OGT Monoclonal Antibody for WB
Cat No. CL488-66823

Print datasheet

CloneNo. 2B2A6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66823-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66823 targets OGT in WB applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen OGT fusion protein Ag28402 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Synonyms
Calculated Molecular Weight 1046 aa, 117 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC014434
Gene Symbol OGT
Gene ID (NCBI) 8473
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15294
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 OGT antibody CL488-66823Download protocol
Standard Protocols
Click here to view our Standard Protocols