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验证数据展示

  • WB analysis using CL488-66823

    WB analysis using CL488-66823

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66823 (OGT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated OGT Monoclonal antibody

OGT Monoclonal Antibody for WB
Cat No. CL488-66823

产品说明书

CloneNo. 2B2A6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy OGT Ready-To-Use IHC Kit. OGT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66823-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66823 targets OGT in WB applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat, pig
免疫原 OGT fusion protein Ag28402 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
别名
计算分子量 1046 aa, 117 kDa
观测分子量 110 kDa
GenBank蛋白编号BC014434
基因名称 OGT
Gene ID (NCBI) 8473
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15294
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 OGT antibody CL488-66823Download protocol
Standard Protocols
Click here to view our Standard Protocols