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OLIG2 Polyclonal antibody, PBS Only

OLIG2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25754-1-PBS

Print datasheet

Synonyms

OLIG 2, Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1

验证数据展示

  • IHC staining of human gliomas using 25754-1-AP (same clone as 25754-1-PBS)

    IHC staining of human gliomas using 25754-1-AP (same clone as 25754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OLIG2 antibody included
  • WB analysis of rat brain using 25754-1-AP (same clone as 25754-1-PBS)

    WB analysis of rat brain using 25754-1-AP (same clone as 25754-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

    IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

    IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy OLIG2 Ready-To-Use IHC Kit. OLIG2 primary antibody included.

产品信息

25754-1-PBS targets OLIG2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OLIG2 fusion protein Ag22642 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oligodendrocyte lineage transcription factor 2
Synonyms OLIG 2, Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC047511
Gene Symbol OLIG2
Gene ID (NCBI) 10215
RRIDAB_2880226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13516
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.