验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
66513-1-Ig targets OLIG2 (for WB only) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | OLIG2 (for WB only) fusion protein Ag18838 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | oligodendrocyte lineage transcription factor 2 |
| 别名 | BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 35-39 kDa |
| GenBank蛋白编号 | BC047511 |
| 基因名称 | OLIG2 |
| Gene ID (NCBI) | 10215 |
| RRID | AB_2881876 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13516 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OLIG2 (for WB only) antibody 66513-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK | ||
Cell Biochem Funct Ring finger protein 216 loss-of-function induces white matter hyperintensities by inhibiting oligodendroglia proliferation | ||
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation | ||
Commun Biol Selenoprotein W modulates tau homeostasis in an Alzheimer's disease mouse model |
