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OPA1 Monoclonal antibody
OPA1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, pig, rat and More (2)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B2D8
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, pig brain tissue, HeLa cells, HepG2 cells, Y79 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66583-1-Ig targets OPA1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, mouse, pig, rat |
| Cited Reactivity | human, mouse, rat, fish |
| Immunogen | OPA1 fusion protein Ag26868 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | optic atrophy 1 (autosomal dominant) |
| Synonyms | KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1 |
| Calculated Molecular Weight | 960 aa, 112 kDa |
| Observed Molecular Weight | 100 kDa and 80-90 kDa |
| GenBank Accession Number | BC075805 |
| Gene Symbol | OPA1 |
| Gene ID (NCBI) | 4976 |
| RRID | AB_2881943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OPA1 antibody 66583-1-Ig | Download protocol |
| IHC protocol for OPA1 antibody 66583-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Pyrroloquinoline Quinone Alleviates Mitochondria Damage in Radiation-Induced Lung Injury in a MOTS-c-Dependent Manner | ||
J Physiol Sci Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart | ||
Behav Brain Res Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice. | ||
Biochim Biophys Acta Mol Basis Dis SENP1 prevents high fat diet-induced non-alcoholic fatty liver diseases by regulating mitochondrial dynamics | ||
Biochim Biophys Acta Mol Basis Dis Electroacupuncture pretreatment maintains mitochondrial quality control via HO-1/MIC60 signaling pathway to alleviate endotoxin-induced acute lung injury |