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Host
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Species Reactivity
0
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0
Conjugate
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验证数据展示

  • WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

    WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

  • WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

    WB analysis using 66583-1-Ig (same clone as 66583-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66583-1-Ig (same clone as 66583-1-PBS)

    WB analysis of HEK-293 using 66583-1-Ig (same clone as 66583-1-PBS)

    WB result of OPA1 antibody (66583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

    IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

    IHC staining of mouse brain using 66583-1-Ig (same clone as 66583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66583-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

OPA1 Monoclonal antibody, PBS Only

OPA1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66583-1-PBS

产品说明书

CloneNo. 1B2D8

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, pig, rat

应用

WB, IHC, ELISA

别名

FLJ12460, KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66583-1-PBS targets OPA1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, pig, rat
免疫原 OPA1 fusion protein Ag26868 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 optic atrophy 1 (autosomal dominant)
别名 FLJ12460, KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1
计算分子量 960 aa, 112 kDa
观测分子量 100 kDa and 80-90 kDa
GenBank蛋白编号BC075805
基因名称 OPA1
Gene ID (NCBI) 4976
RRIDAB_2881943
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60313
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.