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Osteopontin Polyclonal antibody
Osteopontin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)
Conjugate
Unconjugated
323
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells, C2C12 cell, HepG2 cells, mouse kidney tissue |
Positive IHC detected in | mouse kidney tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HEK-293 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22952-1-AP targets Osteopontin in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP, ELISA |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, bovine |
Immunogen | Osteopontin fusion protein Ag19216 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | secreted phosphoprotein 1 |
Synonyms | SPP1, PSEC0156, OPN, ETA 1, BSPI |
Calculated Molecular Weight | 314 aa, 35 kDa |
Observed Molecular Weight | 70 kDa, 44-66 kDa |
GenBank Accession Number | BC007016 |
Gene Symbol | Osteopontin |
Gene ID (NCBI) | 6696 |
RRID | AB_2783651 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P10451 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Osteopontin antibody 22952-1-AP | Download protocol |
IHC protocol for Osteopontin antibody 22952-1-AP | Download protocol |
IF protocol for Osteopontin antibody 22952-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
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