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Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22952-1-AP

Print datasheet

Synonyms

SPP1, PSEC0156, OPN, ETA 1, BSPI



经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, C2C12 cell, HepG2 cells, mouse kidney tissue
Positive IHC detected inmouse kidney tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Osteopontin/SPP1 Ready-To-Use IHC Kit. Osteopontin/SPP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22952-1-AP targets Osteopontin in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, bovine
Immunogen Osteopontin fusion protein Ag19216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secreted phosphoprotein 1
Synonyms SPP1, PSEC0156, OPN, ETA 1, BSPI
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 70 kDa, 44-66 kDa
GenBank Accession NumberBC007016
Gene Symbol Osteopontin
Gene ID (NCBI) 6696
RRIDAB_2783651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10451
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).

实验方案

Product Specific Protocols
WB protocol for Osteopontin antibody 22952-1-APDownload protocol
IHC protocol for Osteopontin antibody 22952-1-APDownload protocol
IF protocol for Osteopontin antibody 22952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Circulation

Carbonylation of Runx2 at K176 by 4-Hydroxynonenal Accelerates Vascular Calcification

Authors - Xiaoxuan Zhai
mouseIF

Adv Mater

Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects

Authors - Congyang Mao
humanIF

Cell Stem Cell

Decoding the temporal and regional specification of microglia in the developing human brain.

Authors - Yanxin Li
rat,humanIF

Bioact Mater

Urchin-like multiscale structured fluorinated hydroxyapatite as versatile filler for caries restoration dental resin composites

Authors - Shuning Zhang
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
mouseIF

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian