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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-22952

Print datasheet

Synonyms

SPP1, PSEC0156, OPN, ETA 1, BSPI

验证数据展示

  • FC experiment of HepG2 using CL647-22952

    FC experiment of HepG2 using CL647-22952

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Osteopontin (CL647-22952) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Osteopontin/SPP1 Ready-To-Use IHC Kit. Osteopontin/SPP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22952-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-22952 targets Osteopontin in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Osteopontin fusion protein Ag19216 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secreted phosphoprotein 1
Synonyms SPP1, PSEC0156, OPN, ETA 1, BSPI
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 70 kDa, 44-66 kDa
GenBank Accession NumberBC007016
Gene Symbol Osteopontin
Gene ID (NCBI) 6696
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10451
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Osteopontin antibody CL647-22952Download protocol
Standard Protocols
Click here to view our Standard Protocols