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  • Featured Product
  • KD/KO Validated

OPTN Monoclonal antibody, PBS Only

OPTN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6C1H4

Cat no : 60293-1-PBS

Print datasheet

Synonyms

Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein

验证数据展示

  • WB analysis of human heart using 60293-1-Ig (same clone as 60293-1-PBS)

    WB analysis of human heart using 60293-1-Ig (same clone as 60293-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60293-1-Ig (OPTN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 60293-1-Ig (same clone as 60293-1-PBS)

    IF Staining of MCF-7 using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (60293-1-Ig, Clone: 6C1H4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

产品信息

60293-1-PBS targets OPTN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen OPTN fusion protein Ag1272 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name optineurin
Synonyms Optineurin, FIP2, FIP 2, FAA4, E3-14.7K-interacting protein
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 70-74 kDa
GenBank Accession NumberBC013876
Gene Symbol OPTN
Gene ID (NCBI) 10133
RRIDAB_2881408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96CV9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.