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  • Featured Product
  • KD/KO Validated

OPTN Monoclonal antibody, PBS Only

OPTN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6C1H4

Cat no : 60293-1-PBS

Print datasheet

Synonyms

AAT4, E3 14.7K interacting protein, FAA4, FIP 2, FIP2, GLC1E, HIP 7, HIP7, Huntingtin yeast partner L, HYPL, NEMO related protein, NRP, optineurin, OPTN

验证数据展示

  • WB analysis of human heart using 60293-1-Ig (same clone as 60293-1-PBS)

    WB analysis of human heart using 60293-1-Ig (same clone as 60293-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60293-1-Ig (OPTN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    IHC staining of human pancreas cancer using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60293-1-Ig (same clone as 60293-1-PBS)

    IF Staining of HepG2 using 60293-1-Ig (same clone as 60293-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60293-1-Ig (OPTN antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60293-1-PBS in a different storage buffer formulation.

产品信息

60293-1-PBS targets OPTN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen OPTN fusion protein Ag1272 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name optineurin
Synonyms AAT4, E3 14.7K interacting protein, FAA4, FIP 2, FIP2, GLC1E, HIP 7, HIP7, Huntingtin yeast partner L, HYPL, NEMO related protein, NRP, optineurin, OPTN
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 70-74 kDa
GenBank Accession NumberBC013876
Gene Symbol OPTN
Gene ID (NCBI) 10133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96CV9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.