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验证数据展示

  • WB analysis of human brain using Biotin-60293

    WB analysis of human brain using Biotin-60293

    Fetal human brain was subjected to SDS-PAGE followed by western blot with Biotin-60293 (biotin labeled OPTN monoclonal antibody) at dilution of 1:1000. Avidin-HRP was used instead of secondary antibody.

  • IHC staining of human pancreas cancer using Biotin-60293

    IHC staining of human pancreas cancer using Biotin-60293

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using Biotin-60293 (OPTN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

Biotin-conjugated OPTN/Optineurin Monoclonal antibody

OPTN/Optineurin Monoclonal Antibody for WB, IHC
Cat No. Biotin-60293

Print datasheet

CloneNo. 6C1H4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC

Synonyms

Optineurin, OPTN, FIP2, FIP 2, FAA4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-60293 targets OPTN/Optineurin in WB, IHC applications and shows reactivity with human samples.

Tested Applications WB, IHC Application Description
Tested Reactivity human
Immunogen OPTN/Optineurin fusion protein Ag1272 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name optineurin
Synonyms Optineurin, OPTN, FIP2, FIP 2, FAA4
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 70-74 kDa
GenBank Accession NumberBC013876
Gene Symbol OPTN
Gene ID (NCBI) 10133
RRIDAB_2883065
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96CV9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. This antibody is conjugated with Biotin.

实验方案

Product Specific Protocols
IHC protocol for Biotin OPTN/Optineurin antibody Biotin-60293Download protocol
Standard Protocols
Click here to view our Standard Protocols