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Biotin-conjugated OPTN/Optineurin Monoclonal antibody

OPTN/Optineurin Monoclonal Antibody for WB, IHC
Cat No. Biotin-60293

产品说明书

CloneNo. 6C1H4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC

Optineurin, OPTN, FIP2, FIP 2, FAA4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman brain
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-60293 targets OPTN/Optineurin in WB, IHC applications and shows reactivity with human samples.

经测试应用 WB, IHC Application Description
经测试反应性 human
免疫原 OPTN/Optineurin fusion protein Ag1272 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 optineurin
别名 Optineurin, OPTN, FIP2, FIP 2, FAA4
计算分子量 66 kDa
观测分子量 70-74 kDa
GenBank蛋白编号BC013876
基因名称 OPTN
Gene ID (NCBI) 10133
RRIDAB_2883065
偶联类型 Biotin
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96CV9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. This antibody is conjugated with Biotin.

实验方案

Product Specific Protocols
IHC protocol for Biotin OPTN/Optineurin antibody Biotin-60293Download protocol
Standard Protocols
Click here to view our Standard Protocols
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