ORAI1 Monoclonal antibody

ORAI1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3C12F9

Cat no : 66223-1-Ig

Print datasheet

Synonyms

ORAI1



经过测试的应用

Positive WB detected inHL-60 cells, 4T1 cells, A375 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, pig thymus tissue, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66223-1-Ig targets ORAI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, monkey, bovine
Immunogen ORAI1 fusion protein Ag18050 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ORAI calcium release-activated calcium modulator 1
Synonyms ORAI1
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC015369
Gene Symbol ORAI1
Gene ID (NCBI) 84876
RRIDAB_2881614
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96D31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

实验方案

Product Specific Protocols
WB protocol for ORAI1 antibody 66223-1-IgDownload protocol
IF protocol for ORAI1 antibody 66223-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang
mouseWB

Acta Pharmacol Sin

Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice

Authors - Xiao Li
humanWB,IF

Oncogenesis

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth

Authors - Chunming Gu
humanWB

Int J Mol Sci

Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y

Authors - Ilaria Nigro
mouseWB

Front Cell Dev Biol

Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling.

Authors - Yuan Cao
mouseWB

J Innate Immun

Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery.

Authors - Yunzhi Liu