验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, 4T1 cells, A375 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, pig thymus tissue, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:30000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66223-1-Ig targets ORAI1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, monkey, bovine |
| 免疫原 | ORAI1 fusion protein Ag18050 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ORAI calcium release-activated calcium modulator 1 |
| 别名 | ORAI1 |
| 计算分子量 | 301 aa, 33 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC015369 |
| 基因名称 | ORAI1 |
| Gene ID (NCBI) | 84876 |
| RRID | AB_2881614 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96D31 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ORAI1 antibody 66223-1-Ig | Download protocol |
| IF protocol for ORAI1 antibody 66223-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats | ||
Acta Pharmacol Sin Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice | ||
Oncogenesis Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth | ||
Int J Mol Sci Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y | ||
Front Cell Dev Biol Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca2+/NFATc3 Signaling. | ||
J Innate Immun Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery. |
