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ORAI1 Monoclonal antibody, PBS Only

ORAI1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12F9

Cat no : 66223-1-PBS

Print datasheet

Synonyms

ORAI1

验证数据展示

  • WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

    IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

产品信息

66223-1-PBS targets ORAI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ORAI1 fusion protein Ag18050 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ORAI calcium release-activated calcium modulator 1
Synonyms ORAI1
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC015369
Gene Symbol ORAI1
Gene ID (NCBI) 84876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96D31
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.