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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

    IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

ORAI1 Monoclonal antibody, PBS Only

ORAI1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66223-1-PBS

产品说明书

CloneNo. 3C12F9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, Indirect ELISA

别名

ORAI1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66223-1-PBS targets ORAI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ORAI1 fusion protein Ag18050 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ORAI calcium release-activated calcium modulator 1
别名 ORAI1
计算分子量 301 aa, 33 kDa
观测分子量 32 kDa
GenBank蛋白编号BC015369
基因名称 ORAI1
Gene ID (NCBI) 84876
RRIDAB_2881614
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96D31
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.