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验证数据展示

  • IF Staining of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    IF Staining of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OS9 antibody (82932-2-RR, Clone: 230149C6 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    FC experiment of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-2-RR, Clone:230149C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82932-2-RR

    Affinity and Kinetic Characterization of 82932-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-2-RR against Human OS9 were performed. The affinity constant is below 1 pM.

OS9 Recombinant antibody, PBS Only

OS9 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82932-2-PBS

产品说明书

CloneNo. 230149C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Protein OS-9, Amplified in osteosarcoma 9, 230149C6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82932-2-PBS targets OS9 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 OS9 fusion protein Ag0106 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 osteosarcoma amplified 9, endoplasmic reticulum associated protein
别名 Protein OS-9, Amplified in osteosarcoma 9, 230149C6
计算分子量 76 kDa
GenBank蛋白编号BC000532
基因名称 OS9
Gene ID (NCBI) 10956
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13438
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.