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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • IF Staining of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    IF Staining of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OS9 antibody (82932-2-RR, Clone: 230149C6 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    FC experiment of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-2-RR, Clone:230149C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82932-2-RR

    Affinity and Kinetic Characterization of 82932-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-2-RR against Human OS9 were performed. The affinity constant is below 1 pM.

OS9 Recombinant antibody, PBS Only

OS9 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82932-2-PBS
CloneNo. 230149C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Protein OS-9, Amplified in osteosarcoma 9, 230149C6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82932-2-PBS targets OS9 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen OS9 fusion protein Ag0106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Synonyms Protein OS-9, Amplified in osteosarcoma 9, 230149C6
Calculated Molecular Weight 76 kDa
GenBank Accession NumberBC000532
Gene Symbol OS9
Gene ID (NCBI) 10956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13438
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.