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OS9 Recombinant antibody

OS9 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230149C6

Cat no : 82932-2-RR

Print datasheet

Synonyms

Protein OS-9, Amplified in osteosarcoma 9, 230149C6

验证数据展示

  • IF Staining of HeLa using 82932-2-RR

    IF Staining of HeLa using 82932-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OS9 antibody (82932-2-RR, Clone: 230149C6 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 82932-2-RR

    FC experiment of HeLa using 82932-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-2-RR, Clone:230149C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82932-2-RR

    Affinity and Kinetic Characterization of 82932-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82932-2-RR against Human OS9 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82932-2-RR targets OS9 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen OS9 fusion protein Ag0106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name osteosarcoma amplified 9, endoplasmic reticulum associated protein
Synonyms Protein OS-9, Amplified in osteosarcoma 9, 230149C6
Calculated Molecular Weight 76 kDa
GenBank Accession NumberBC000532
Gene Symbol OS9
Gene ID (NCBI) 10956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13438
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.

实验方案

Product Specific Protocols
IF protocol for OS9 antibody 82932-2-RRDownload protocol
FC protocol for OS9 antibody 82932-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols