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OSMR Recombinant antibody

OSMR Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241865C8

Cat no : 84555-2-RR

Print datasheet

Synonyms

Interleukin-31 receptor subunit beta, IL-31R-beta, IL-31RB, IL-31R subunit beta, IL-31 receptor subunit beta

验证数据展示

  • WB analysis using 84555-2-RR

    WB analysis using 84555-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84555-2-RR (OSMR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 84555-2-RR

    IHC staining of mouse cerebellum using 84555-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84555-2-RR (OSMR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, Raji cells, A375 cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84555-2-RR targets OSMR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen OSMR fusion protein Ag1426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name oncostatin M receptor
Synonyms Interleukin-31 receptor subunit beta, IL-31R-beta, IL-31RB, IL-31R subunit beta, IL-31 receptor subunit beta
Calculated Molecular Weight 110 kDa
Observed Molecular Weight~180 kDa
GenBank Accession NumberBC010943
Gene Symbol OSMR
Gene ID (NCBI) 9180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99650
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Oncostatin M (OSM) is a member of interleukin-6 family cytokines that modulates proliferation and differentiation in numerous cells (PMID: 22982441). OSM signals via a receptor complex comprising gp130 and the OSMR (also called OSMRβ) (PMID: 8999038). OSMR is a single-pass membrane protein that belongs to the type I cytokine receptor family. OSMR mRNA is expressed at relatively high levels in all neural cells and fibroblast, epithelial, and various tumor cell lines (PMID: 8999038). It has also been shown that OSMR associates with IL31RA to form the heterodimeric IL31 receptor (PMID: 15184896). OMSR has a calculated molecular weight of 110 kDa, with an apparent molecular weight of 150-180 kDa (PMID: 8999038; 17028186; 39266541; 34644107). A 70 kDa soluble form can be detected by western blot (PMID: 17028186).

实验方案

Product Specific Protocols
WB protocol for OSMR antibody 84555-2-RRDownload protocol
IHC protocol for OSMR antibody 84555-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols