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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of HeLa using 84717-1-RR (same clone as 84717-1-PBS)

    WB analysis of HeLa using 84717-1-RR (same clone as 84717-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

  • WB analysis of Neuro-2a using 84717-1-RR (same clone as 84717-1-PBS)

    WB analysis of Neuro-2a using 84717-1-RR (same clone as 84717-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01521-2

    Cytometric bead array standard curve of MP01521-2

    Cytometric bead array standard curve of MP01521-2, OTUD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84717-1-PBS. Detection antibody: 84717-2-PBS. Standard: Ag30425. Range: 0.781-50 ng/mL.

  • Affinity and Kinetic Characterization of 84717-1-RR

    Affinity and Kinetic Characterization of 84717-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84717-1-RR against Human OTUD3 were performed. The affinity constant is below 1 pM.

OTUD3 Recombinant antibody, PBS Only (Capture)

OTUD3 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84717-1-PBS
CloneNo. 242202B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

RP11 460G22.1, OTU domain containing 3, KIAA0459, DUBA4, 242202B11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84717-1-PBS targets OTUD3 as part of a matched antibody pair:

MP01521-2: 84717-1-PBS capture and 84717-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen OTUD3 fusion protein Ag30425 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name OTU domain containing 3
Synonyms RP11 460G22.1, OTU domain containing 3, KIAA0459, DUBA4, 242202B11
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberNM_015207
Gene Symbol OTUD3
Gene ID (NCBI) 23252
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5T2D3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.