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验证数据展示

  • WB analysis of HeLa using 84717-1-RR

    WB analysis of HeLa using 84717-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of Neuro-2a using 84717-1-RR

    WB analysis of Neuro-2a using 84717-1-RR

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84717-1-RR

    Affinity and Kinetic Characterization of 84717-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84717-1-RR against Human OTUD3 were performed. The affinity constant is below 1 pM.

OTUD3 Recombinant antibody

OTUD3 Recombinant Antibody for WB, ELISA
Cat No. 84717-1-RR

产品说明书

CloneNo. 242202B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

RP11 460G22.1, OTU domain containing 3, KIAA0459, DUBA4, 242202B11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Neuro-2a cells, HepG2 cells, MCF-7 cells, HCT 116 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84717-1-RR targets OTUD3 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 OTUD3 fusion protein Ag30425 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 OTU domain containing 3
别名 RP11 460G22.1, OTU domain containing 3, KIAA0459, DUBA4, 242202B11
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号NM_015207
基因名称 OTUD3
Gene ID (NCBI) 23252
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5T2D3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.

实验方案

Product Specific Protocols
WB protocol for OTUD3 antibody 84717-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols