验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Neuro-2a cells, HepG2 cells, MCF-7 cells, HCT 116 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84717-1-RR targets OTUD3 in WB, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | OTUD3 fusion protein Ag30425 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | OTU domain containing 3 |
Synonyms | RP11 460G22.1, OTU domain containing 3, KIAA0459, DUBA4, 242202B11 |
Calculated Molecular Weight | 45 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | NM_015207 |
Gene Symbol | OTUD3 |
Gene ID (NCBI) | 23252 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q5T2D3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.
实验方案
Product Specific Protocols | |
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WB protocol for OTUD3 antibody 84717-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |