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OX40L/TNFSF4 Recombinant antibody

OX40L/TNFSF4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241813A6

Cat no : 82794-7-RR

Print datasheet

Synonyms

OX40L, TNFSF4, TNFSF4/CD252, TNFSF 4, OX40 ligand

验证数据展示

  • WB analysis of HUVEC using 82794-7-RR

    WB analysis of HUVEC using 82794-7-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MJ using 82794-7-RR

    IF Staining of MJ using 82794-7-RR

    Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82794-7-RR

    Affinity and Kinetic Characterization of 82794-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82794-7-RR against Human OX40L/TNFSF4 were performed. The affinity constant is 13.8 pM.

经过测试的应用

Positive WB detected inHUVEC cells
Positive IF/ICC detected inMJ cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82794-7-RR targets OX40L/TNFSF4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 4
Synonyms OX40L, TNFSF4, TNFSF4/CD252, TNFSF 4, OX40 ligand
Observed Molecular Weight30 kDa
GenBank Accession NumberNM_003326.5
Gene Symbol OX40L/TNFSF4
Gene ID (NCBI) 7292
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23510
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OX40L/TNFSF4 antibody 82794-7-RRDownload protocol
IF protocol for OX40L/TNFSF4 antibody 82794-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols