验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, NIH/3T3 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21055-1-AP targets OXA1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | OXA1L fusion protein Ag13819 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oxidase (cytochrome c) assembly 1-like |
| 别名 | Oxidase assembly 1-like protein, OXA1-like protein, OXA1 |
| 计算分子量 | 437 aa, 49 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC001669 |
| 基因名称 | OXA1L |
| Gene ID (NCBI) | 5018 |
| RRID | AB_10695769 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15070 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OXA1L antibody 21055-1-AP | Download protocol |
| IHC protocol for OXA1L antibody 21055-1-AP | Download protocol |
| IF protocol for OXA1L antibody 21055-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation. | ||
Am J Hum Genet Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number. | ||
EMBO Mol Med OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect.
| ||
Aging (Albany NY) Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation. | ||
J Cell Sci Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis |
