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验证数据展示

  • WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

    WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

    IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

    IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OXCT1 antibody included
  • IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    FC experiment of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

OXCT1 Monoclonal antibody, PBS Only

OXCT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67836-1-PBS

产品说明书

CloneNo. 1G1B9

宿主/亚型

Mouse / IgG1

种属反应性

rabbit, pig, rat, mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.

产品信息

67836-1-PBS targets OXCT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 rabbit, pig, rat, mouse, human
免疫原 OXCT1 fusion protein Ag24792 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 3-oxoacid CoA transferase 1
别名 SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1
计算分子量 520 aa, 56 kDa
观测分子量52-56 kDa
GenBank蛋白编号BC009001
基因名称 OXCT1
Gene ID (NCBI) 5019
RRIDAB_2918598
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP55809
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)