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  • Featured Product
  • KD/KO Validated

OXCT1 Monoclonal antibody, PBS Only

OXCT1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1B9

Cat No : 67836-1-PBS

Print datasheet

Synonyms

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

验证数据展示

  • WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

    WB analysis of rat heart using 67836-1-Ig (same clone as 67836-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    WB analysis of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

    IF Staining of MCF-7 using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

    IHC staining of rat brain using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OXCT1 antibody included
  • IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    IF Staining of HeLa using 67836-1-Ig (same clone as 67836-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67836-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.

产品信息

67836-1-PBS targets OXCT1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen OXCT1 fusion protein Ag24792 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 3-oxoacid CoA transferase 1
Synonyms 3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s
Calculated Molecular Weight 520 aa, 56 kDa
Observed Molecular Weight52-56 kDa
GenBank Accession NumberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55809
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)