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OXCT1 Monoclonal antibody

OXCT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G1B9

Cat no : 67836-1-Ig

Print datasheet

Synonyms

SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1

验证数据展示

  • WB analysis of rat heart using 67836-1-Ig

    WB analysis of rat heart using 67836-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67836-1-Ig

    WB analysis of HeLa using 67836-1-Ig

    WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

  • IF Staining of MCF-7 using 67836-1-Ig

    IF Staining of MCF-7 using 67836-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red).

  • IHC staining of rat brain using 67836-1-Ig

    IHC staining of rat brain using 67836-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with OXCT1 antibody included
  • IF Staining of HeLa using 67836-1-Ig

    IF Staining of HeLa using 67836-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 67836-1-Ig

    IF Staining of HeLa using 67836-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 67836-1-Ig

    FC experiment of HeLa using 67836-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inrat heart tissue, HeLa cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67836-1-Ig targets OXCT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Immunogen OXCT1 fusion protein Ag24792 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 3-oxoacid CoA transferase 1
Synonyms SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1
Calculated Molecular Weight 520 aa, 56 kDa
Observed Molecular Weight52-56 kDa
GenBank Accession NumberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
RRIDAB_2918598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

实验方案

Product Specific Protocols
WB protocol for OXCT1 antibody 67836-1-IgDownload protocol
IHC protocol for OXCT1 antibody 67836-1-IgDownload protocol
IF protocol for OXCT1 antibody 67836-1-IgDownload protocol
FC protocol for OXCT1 antibody 67836-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Med

Induction of resistance to neurotrophic tropomyosin-receptor kinase inhibitors by HMGCS2 via a mevalonate pathway

Authors - Yasuhiro Kato
View Article