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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated OXCT1 Monoclonal antibody

OXCT1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1B9

Cat no : CL488-67836

Print datasheet

Synonyms

SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1

验证数据展示

  • IF Staining of HeLa using CL488-67836

    IF Staining of HeLa using CL488-67836

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OXCT1 antibody (CL488-67836, Clone: 1G1B9 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67836

    FC experiment of HeLa using CL488-67836

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human OXCT1 (CL488-67836, Clone:1G1B9) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67836-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67836 targets OXCT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen OXCT1 fusion protein Ag24792 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 3-oxoacid CoA transferase 1
Synonyms SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1, 3 oxoacid CoA transferase 1
Calculated Molecular Weight 520 aa, 56 kDa
GenBank Accession NumberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55809
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 OXCT1 antibody CL488-67836Download protocol
FC protocol for CL Plus 488 OXCT1 antibody CL488-67836Download protocol
Standard Protocols
Click here to view our Standard Protocols