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OXCT1 Recombinant antibody, PBS Only

OXCT1 Recombinant Antibody for WB,Indirect ELISA,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

4K12

Cat No : 81011-1-PBS

Print datasheet

Synonyms

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

验证数据展示

  • WB analysis using 81011-1-RR (same clone as 81011-1-PBS)

    WB analysis using 81011-1-RR (same clone as 81011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81011-1-RR (OXCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    WB analysis of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    WB result of OXCT1 antibody (81011-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells. This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    IF Staining of HeLa using 81011-1-RR (same clone as 81011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

  • IF Staining of SKOV-3 using 81011-1-RR (same clone as 81011-1-PBS)

    IF Staining of SKOV-3 using 81011-1-RR (same clone as 81011-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81011-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.

产品信息

81011-1-PBS targets OXCT1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen OXCT1 fusion protein Ag2818 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 3-oxoacid CoA transferase 1
Synonyms 3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s
Calculated Molecular Weight 520 aa, 56 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55809
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)