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验证数据展示

  • WB analysis using 81011-1-RR

    WB analysis using 81011-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81011-1-RR (OXCT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 81011-1-RR

    WB analysis of HeLa using 81011-1-RR

    WB result of OXCT1 antibody (81011-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

  • IF Staining of HeLa using 81011-1-RR

    IF Staining of HeLa using 81011-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of SKOV-3 using 81011-1-RR

    IF Staining of SKOV-3 using 81011-1-RR

    Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using OXCT1 antibody (81011-1-RR, Clone: 4K12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

OXCT1 Recombinant antibody

OXCT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81011-1-RR

产品说明书

CloneNo. 4K12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, 4K12, EC:2.8.3.5, OXCT

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, mouse skeletal muscle, mouse brain, rat brain, mouse heart, rat heart
Positive IF/ICC detected inHeLa cells, SKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81011-1-RR targets OXCT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OXCT1 fusion protein Ag2818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-oxoacid CoA transferase 1
别名 3 oxoacid CoA transferase 1, 3-oxoacid CoA-transferase 1, 4K12, EC:2.8.3.5, OXCT
计算分子量 520 aa, 56 kDa
观测分子量56 kDa
GenBank蛋白编号BC009001
基因名称 OXCT1
Gene ID (NCBI) 5019
RRIDAB_2918924
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55809
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

实验方案

Product Specific Protocols
WB protocol for OXCT1 antibody 81011-1-RRDownload protocol
IF protocol for OXCT1 antibody 81011-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols